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| Registros recuperados: 63 | |
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| Bustabad, E. A.; Garcia, G; Rodriguez-pardo, L.; Farina, J.; Perrot, H.; Gabrielli, C.; Bucur, B.; Lazerges, M.; Rose, D.; Compere, Chantal; Arnau, A.. |
| This work deals with the development of a high sensibility DNA biosensor based on a 50MHz Quartz Crystal Microbalance (QCM) Oscillator Electronic Circuit. The designed QCM oscillator sensor is able to detect the presence of complementary DNAs in a solution that match the sequence on a given strand in function of the changes in the output frequency of the oscillator. The design is adapted so that the Barkhausen condition is satisfied even when the quartz is immerged in liquid media. Also a comparative study of the developed 50MHz biosensor in front of a QCM oscillator with smaller frequency is carried out, with object of checking if the sensibility of the oscillator increases, allowing to detect smaller concentrations of the complementary DNA. |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00021/13198/10574.pdf |
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| Leroy, Celine; Compere, Chantal; Delbarre Ladrat, Christine; Raguenes, Gerard; Ghillebaert, François; Combes, Didier. |
| Une nouvelle bactérie marine du genre Pseudoalteromonas sp., D41, formant rapidement des biofilms sur tout type de matériau (verre, acier inoxydable, Téflon) a été isolée en Baie de Brest. L’objectif de ce présent travail est de caractériser et décrire cette bactérie capable de produire des exopolymères constitués en majorité de protéines. Le rôle des protéines sécrétées, dans les mécanismes d’adhésion, est discuté. |
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| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00088/19950/17617.pdf |
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| Dheilly, Alexandra; Soum-soutera, Emmanuelle; Klein, Geraldine; Bazire, Alexis; Compere, Chantal; Haras, Dominique; Dufour, Aurelie. |
| Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00004/11530/11317.pdf |
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| Briand, Elisabeth; Salmain, M; Compere, Chantal; Pradier, C. |
| Two antibody immobilisation procedures were compared to set up an immunosensor for goat anti-rabbit immunoglobulin (anti-rIgG), i.e. rIgG covalently bound or immobilised via affinity to protein A (PrA). In both cases, the first layer of protein was covalently bound to a mixed self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) on a gold surface. The elaboration of the sensitive surfaces, as well as their selectivity and sensitivity were studied step by step by polarization modulation-reflection absorption infra-red spectroscopy (PM-RAIRS) and quartz crystal trticrobalance (QCM) with impedance measurement. QCM measurements showed that the viscoelastic properties of the antibody layer were markedly modified during the... |
| Tipo: Text |
Palavras-chave: RIgG immobilisation; PM RAIRS; QCM; Immunosensors. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3580.pdf |
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| Compere, Chantal; Le Bozec, Nathalie. |
| In this paper, investigations performed in natural and artificial seawater on stainless steels will be presented. They concerned studies on: biofilm formation, passive layers composition, electrochemical behaviour, localised corrosion and the evolution of these different parameters as a function of ageing time. According to literature surveys, the different aspects will be discussed. Some conclusions will be drawn concerning the actual knowledge on the behaviour of stainless steels in seawater. |
| Tipo: Text |
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| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00089/19989/17653.pdf |
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| Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain. |
| Bacterial biofilms, frequently in association with algae, protozoa and fungi, are found on all submerged structures in the marine environment. Biofilms are responsible for a range of surface‐associated and diffusible signals, which may moderate the settling behaviour of cells, spores and larvae. Thus, marine microorganisms are a new source of bioactive compounds, which enhance or inhibit the settlement of organisms. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00089/20005/17667.pdf |
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| Compere, Chantal. |
| Introduction: Almost all submerged surfaces in the marine environment are subject to developing an organic film, with the exception of some living organisms, such as sponges, which do not undergo any great colonization, for no currently-known scientific reason. The presence of this biofilm is at the source of many problems in the oceanographic field and for marine business. This article introduces some drawbacks relating to the existence of this fouling and elaborates upon the main studies conducted at IFREMER concerning the most-used methods for combating it. However, the anti-fouling agents that are generally used for protecting facilities are toxic and can have disastrous consequences on the marine environment's fauna and flora. Moreover, the use of... |
| Tipo: Text |
Palavras-chave: Biofilm; Seawater. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-1698.pdf |
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| Delauney, Laurent; Compere, Chantal; Lehaitre, Michel. |
| These days, many marine autonomous environment monitoring networks are set up in the world. These systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or underwater oceanographic structures. These systems commonly use various sensors to measure parameters such as dissolved oxygen, turbidity, conductivity, pH or fluorescence. Emphasis has to be put on the long term quality of measurements, yet sensors may face very short-term biofouling effects. Biofouling can disrupt the quality of the measurements, sometimes in less than a week. Many techniques to prevent biofouling on instrumentation are listed and studied by researchers and manufacturers. Very few of them... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00008/11920/8626.pdf |
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| Delauney, Laurent; Compere, Chantal; Lehaitre, Michel. |
| These days, many marine autonomous environment monitoring networks are set up in the world. Such systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or deep sea fix stations. The major goal for these equipments is to provide in real time reliable measurements without costly frequent maintenance. These autonomous monitoring systems are affected by a well known phenomena in seawater condition which is biofouling. Consequently, such system without efficient biofouling protection is hopeless. This protection must be applied to the sensors and to the underwater acoustic communication equipments. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00107/21801/20176.pdf |
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| Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle. |
| The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae. |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf |
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| Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie. |
| Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved. |
| Tipo: Text |
Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf |
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| Anne, Marie-laure; Keirsse, Julie; Nazabal, Virginie; Hyodo, Koji; Inoue, Satoru; Boussard-pledel, Catherine; Lhermite, Herve; Charrier, Joel; Yanakata, Kiyoyuki; Loreal, Olivier; Le Person, Jenny; Colas, Florent; Compere, Chantal; Bureau, Bruno. |
| Due to the remarkable properties of chalcogenide (Chg) glasses, Chg optical waveguides should play a significant role in the development of optical biosensors. This paper describes the fabrication and properties of chalcogenide fibres and planar waveguides. Using optical fibre transparent in the mid-infrared spectral range we have developed a biosensor that can collect information on whole metabolism alterations, rapidly and in situ. Thanks to this sensor it is possible to collect infrared spectra by remote spectroscopy, by simple contact with the sample. In this way, we tried to determine spectral modifications due, on the one hand, to cerebral metabolism alterations caused by a transient focal ischemia in the rat brain and, in the other hand, starvation... |
| Tipo: Text |
Palavras-chave: Planar waveguide; Fibre; Optical sensor; Chalcogenide. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6851.pdf |
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| Klein, Geraldine; Dufour, Alain; Compere, Chantal. |
| Lors d’une immersion en eau de mer, tous les matériaux et structures sont rapidement colonisés par des salissures d’origine biologique : des biofilms. La transformation des propriétés de surface dumatériau et la présence demicro- oumacro-organismes (bactéries, algues, balanes, larves) engendrent des risques accrus de corrosion localisée, la biodétérioration desmatériaux immergés, le blocage des fonctions mécaniques…Afin de contrôler le dépôt et le développement de ces biofilms, la méthode privilégiée est l’application de peintures anti-salissures. Cependant ces revêtements sont généralement toxiques pour l’environnement. C’est pourquoi, les travaux menés actuellement visent à isoler de nouvelles molécules, produites par les bactéries marines, dans le but... |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00351/46213/45910.pdf |
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| Perez Blanco, Eva; Antoine, Elisabeth; Crassous, Marie-pierre; Compere, Chantal. |
| Domoic acid (DA) was first detected in France in mussel flesh in 1998, outbreak that was linked to the presence of Pseudo-nitzschia pseudodelicatissima. Ever since, the phytoplankton monitoring program (REPHY, Réseau de surveillance du Phytoplancton et des Phycotoxines) also monitors DA content in shellfish flesh. Amnesic shellfish poisoning (ASP) has been recurrently detected, and was over the sanitary threshold in the years 2000 and 2004 in Brittany and in 2002 in the Mediterranean coast (Amzil, 2001; Amzil pers. comm.). Toxicity events are usually dominated by several Pseudo-nitzschia species, although different strains might be or not be toxic (Bates et al., 1998). This is why an inventory of the species present in the French coasts and their molecular... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00089/19988/17652.pdf |
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| Registros recuperados: 63 | |
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